cell culture 65 human oscc cell lines scc9  (ATCC)

Journal: Oncology Letters

Article Title: Periostin serves an important role in the pathogenesis of oral squamous cell carcinoma

doi: 10.3892/ol.2018.9660

Figure Lengend Snippet: Periostin expression in OSCC. (A) Representative images of periostin immunohistochemical staining (×200) and cells in the red boxes at ×400, and (B) mRNA expression of periostin examined by RT-qPCR in OSCC and healthy adjacent tissues. (C) Expression of periostin mRNA was examined by RT-qPCR in OSCC cells. (D) Protein expression of periostin was evaluated by western blotting in OSCC tissues and cell lines. *P<0.05 vs. corresponding control group. OSCC, oral squamous cell carcinoma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.

Article Snippet: Cell culture Human OSCC cell lines SCC-9 and SCC-25 (ATCC, Manassas, VA, USA) were used between passages 10 and 20.

Techniques: Expressing, Immunohistochemical staining, Staining, Quantitative RT-PCR, Western Blot, Control, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Oncology Letters

Article Title: Periostin serves an important role in the pathogenesis of oral squamous cell carcinoma

doi: 10.3892/ol.2018.9660

Figure Lengend Snippet: IOD of periostin in OSCC by immunohistochemical staining.

Article Snippet: Cell culture Human OSCC cell lines SCC-9 and SCC-25 (ATCC, Manassas, VA, USA) were used between passages 10 and 20.

Techniques: Immunohistochemical staining, Staining

Journal: Oncology Letters

Article Title: Periostin serves an important role in the pathogenesis of oral squamous cell carcinoma

doi: 10.3892/ol.2018.9660

Figure Lengend Snippet: Association of the clinical characteristics of oral squamous cell carcinoma patients with positive (n=78; 86.7%) and negative (n=12; 13.3%) periostin expression.

Article Snippet: Cell culture Human OSCC cell lines SCC-9 and SCC-25 (ATCC, Manassas, VA, USA) were used between passages 10 and 20.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Periostin serves an important role in the pathogenesis of oral squamous cell carcinoma

doi: 10.3892/ol.2018.9660

Figure Lengend Snippet: Impact of periostin on the growth characteristics of OSCC cells. (A) mRNA and (B) protein levels of periostin were examined by reverse transcription- quantitative polymerase chain reaction and western blotting, respectively, after the cells were transfected with the overexpression plasmid or periostin siRNA. (C) Stimulatory effect of periostin on the proliferation of transfected SCC9 and SCC25 cells, as evaluated by the MTS assay. *P<0.05 vs. corresponding control group. OSCC, oral squamous cell carcinoma; siRNA, small interfering RNA; Ctrl, control.

Article Snippet: Cell culture Human OSCC cell lines SCC-9 and SCC-25 (ATCC, Manassas, VA, USA) were used between passages 10 and 20.

Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Transfection, Over Expression, Plasmid Preparation, MTS Assay, Control, Small Interfering RNA